This book presents a bio-inspired hierarchical control scheme step by step toward developing limbless robots capable of 3D locomotion, fast reflex response, as well as sophisticated reaction to environmental stimuli. This interdisciplinary book introduces how to combine biological concept with locomotion control of limbless robots. The special features of the book include limbless locomotion classification and control, design of biological locomotor and the integration of sensory information into the locomotor using artificial intelligence methods, and on-site demonstrations of limbless locomotion in different scenarios. The book is suitable for readers with engineering background, especially for researchers focused on bio-inspired robots.